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【Objective】 To investigate the effect of sample processing at 56℃ for 30 min on routine examination in Department of Blood Transfusion. 【Methods】 A total of 40 cross matched blood samples submitted by clinical departments of our hospital, were collected, and each sample was equally divided into two. Before and after heating at 56℃ for 30 min, the ABO blood group was detected by manual method and card method (gel card and glass beadle card), antibody titer was detected by coagulant method, and cross-matching was conducted by anti-globulin card method. Chi-square test and Wilcoxon signed rank test were used to compare the differences between the two groups (before and after heating treatment). 【Results】 The blood group detection rates of the experimental group were 100% (40/40), 37.5% (15/40) and 80% (32/40) by manual test tube method, gel card and glass beads card, respectively, P0.05). The matching rate of two groups of samples, cross-matched with corresponding donor samples, was both 100% (40/40) by coagulant method, and 100% (40/40) vs 25% (10/40) respectively by the antiglobulin card method (P<0.01). The other 30 samples in the experimental group presented weak agglutination in the secondary side. 【Conclusion】 The treatment of virus inactivation at 56℃ for 30 min has little effect on blood group identification by test tube method, antibody titer and cross-matching by coagulant method, and reduceds the occupational exposure of staff in Blood Transfusion Department.
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Rhabditis axei is a free-living nematode, which can occasionally invade into humans through drinking and contacting wastewater. It is usually parasitic in the digestive and urinary systems, causing the disease of rhabditelliasis axei. This paper reports a case of R. axei infection found in the urine routine examination.
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Rhabditis axei is a free-living nematode, which can occasionally invade into humans through drinking and contacting wastewater. It is usually parasitic in the digestive and urinary systems, causing the disease of rhabditelliasis axei. This paper reports a case of R. axei infection found in the urine routine examination.
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This paper reports one case of anemia due to hookworm disease.The case was diagnosed through the stool routine examination,in which the hookworm ova were found.It is suggested that the role of stool routine examination in the diagnosis of hookworm disease should be paid much attention.
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Objective To investigate the health effects on practitioners occupationally exposed to acrylonitrile and to provide scientific bases for the study of toxicological effects of acrylonitrile on human bodies. Methods 465 medical practitioners exposed to acrylonitrile and ( a control group of ) 488 medical practitioners unexposed to acrylonitrile were selected .Age, smoking habits , alcoholic habits and other relevant factors are well-considered in selecting these two groups and all of them had lived in Ningbo for at least three years .Blood samples were collected to measure the level of Alanine aminotransferase ( ALT) , aspartate aminotransferase ( AST ) , alkaline phosphatase ( ALP ) , pancreatic acyl transferase (GGT), total bilirubin (STB), blood urea (UREA), blood uric acid (UA), serum creatinine (SCr), total protein ( TP ) , albumin ( Alb ) , globulin ( Glb ) , ratio of Alb to Glb ( A/G ) , white blood cell ( WBC ) , red blood cell ( RBC ) , platelet ( PLT ) and hemoglobin ( Hb ) .These serum biochemical indices of the two groups were compared to find the differences thereof and to examine the effect of work years on various above-mentioned indices for medical practitioners exposed to acrylonitrile . Results The levels of ALT(t=-2.77,P=0.006), AST(t=-5.74,P<0.001), UREA(t=3.51,P<0.001), UA (t=-3.51,P<0.001)and SCr(t=-7.62,P<0.001)for medical practitioners exposed to acrylonitrile were all significantly higher than those for the control group; however, the levels of ALP(t=18.87,P<0.001), Alb(t=6.92,P<0.001), Glb(t=7.99,P<0.001), A/G(t=11.93,P<0.001), and WBC (t=4.48,P<0.001) were all lower than those for the control group , with the exposure time exhibiting positive correlations with ALT(r=0.564,P<0.001)and AST(r=0.493,P<0.001). Conclusion Acrylonitrile has certain health effects on the hepatorenal function as well as the routine blood test results of medical practitioners occupationally exposed to acrylonitrile .
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Objective To study the routine examination of Aconitum sinomontanum Nakai and determine the contents of lappacontine and ranaconitine; To provide basis for establishing the quality standard of Aconitum sinomontanum Nakai.Methods Aconitum sinomontanum Nakai were collected from different areas.A method of TLC was used for qualitative discrimination. The methods in the Chinese Pharmacopoeia were adopted for the determination of moisture content, ash content and extractives. Determination of lappacontine and ranaconitine were performed by HPLC. Results The TLC showed that the spots were clear and the separation was good. Individual provisional standards:the moisture,total ash and acid-insoluble ash content of Aconitum sinomontanum Nakai were not more than 11.0%, 12.0%, and 7.0%, respectively; water soluble and alcohol soluble extractives were not less than 18.2% and 10.6%,respectively.The content of ranaconitine and lappacontine in Aconitum sinomontanum Nakai were not less than 0.125% and 0.815%, respectively. Conclusion The method established by the study is accurate and reliable,and can be used for quality evaluation of Aconitum sinomontanum Nakai.
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Objective To study the routine examination of Aconitum sinomontanum Nakai and determine the contents of lappacontine and ranaconitine; To provide basis for establishing the quality standard of Aconitum sinomontanum Nakai.Methods Aconitum sinomontanum Nakai were collected from different areas.A method of TLC was used for qualitative discrimination. The methods in the Chinese Pharmacopoeia were adopted for the determination of moisture content, ash content and extractives. Determination of lappacontine and ranaconitine were performed by HPLC. Results The TLC showed that the spots were clear and the separation was good. Individual provisional standards:the moisture,total ash and acid-insoluble ash content of Aconitum sinomontanum Nakai were not more than 11.0%, 12.0%, and 7.0%, respectively; water soluble and alcohol soluble extractives were not less than 18.2% and 10.6%,respectively.The content of ranaconitine and lappacontine in Aconitum sinomontanum Nakai were not less than 0.125% and 0.815%, respectively. Conclusion The method established by the study is accurate and reliable,and can be used for quality evaluation of Aconitum sinomontanum Nakai.
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ObjectiveComparingfourgroupsofimmunosuppressivemousemodelsestablishedby cyclophosphamide administration in different doses and periods , we used partial least squares ( PLS) analysis to evaluate the immunosuppressive mouse models comprehensively and select the appropriate way to establish this model .Methods In this experimental study, 58 male KM mice were randomly divided into five groups: normal group (10 mice) were given normal saline daily by i.p.injection, model group 1 (12 mice) was given 40 mg/kg CTX daily by i.p.injection for 10 days, model group 2 (12 mice) was given 80 mg/kg CTX daily by i.p.injection for 3 days, model group 3 (12 mice) was given 40 mg/kg CTX daily by i.p.injection twice for a week, model group 4 (12 mice) was given 50 mg/kg CTX daily by i.p.injection for 7 days.After the injection of cyclophosphamide , the daily metabolic activities were detected everyday such as body weight , water intake , and food intake , organ index and immunological indexes such as blood RT of the model mice were measured as well .Using partial least squares ( PLS) analysis to the models and analyzing the final weight , final anal temperature , organ index , and blood routine examination in order to evaluate the immunosuppressive mouse models comprehensively .Results Compared with the normal group , different dosages of CTX reduced the weight and anal temperature of model mice (P<0.05), the food intake and water intake (P<0.01), and the spleen index and thymus index ( P<0.01 ) .Besides , the amount and percentage of basophilic granulocytes decreased ( P <0.05 ) , and the percentage of MCHC , macrophage went up , as well as the absolute value of WBC and lymphocytes were decreased in the model groups 1, 2, and 4 (P<0.05).According to the PLS analysis, there were significant differences among models 1, 2, and 4 when compared with the normal group , especially the most different in the model group 1.Conclusions After the PLS analysis of different indexes , the optimal way to establish immunosuppressive mouse models is the procedure with 40 mg/kg CTX daily injected i .p.for ten days .Our findings provide experimental evidence for the establishment of immunosuppressive mouse models .
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Objective To preliminarily understand and evaluate the fertility ability of male infertility patients by analyzing the semen routine testing results to assist further clinical diagnosis and treatment .Methods 7 586 cases of semen routine detection re‐sults were performed the retrospective analysis and statistics by classification according to the indexes of semen volume ,pH value , liquefaction time ,sperm density ,sperm activity and sperm deformation rate .Results Among 7 586 cases of semen routine examina‐tion ,there were 1 450 cases(19 .1% ) of semen volume less than 2 .0 mL ;1 372 cases (18 .1% ) of pH 60 min ;989 cases(13 .0% ) of sperm density lower than 20 × 106 /mL ;3 719 cases(49 .0% ) of sperm activity grade a + b less than 50% ;3 431 cases(45 .2% ) of sperm deformation rate less than 15% ;141 cases(1 .9% ) of aspermatism ;1 119 cases (14 .8% ) had no abnormality in each index .Conclusion Semen liquefaction ,sperm motility and sperm deformation rate are the indexes with the highest abnormal rate in the semen routine detection ;the semen routine detection provides the important exper‐imental data for clinical diagnosis and treatment of male infertility ,the objective and correct analysis of the detection result is of great significance to the judgment of male fertility .
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Objective To explore the feasibility of measurement uncertainty in complete blood count using internal quality con-trol(IQC) data associated with Sysmex Network Communication System(SNCS) comparative data .Methods Complete blood count assay including white blood cell(WBC) count ,red blood cell(RBC) count ,hemoglobin(Hb) determination ,hematocrit(HCT) values and platelet(PLT) count were involved to evaluate measurement uncertainty according to the instruction of CNAS-TRL-001 .Meas-urement uncertainty evaluation was established by internal measurement reproducibility using IQC data ,and standard measurement uncertainty by bias using proficiency testing(PT) data and SNCS data ,followed by relative combined standard measurement uncer-tainty and relative expanded measurement uncertainty was calculated .Meanwhile ,the measurement uncertainty was compared by u-sing PT data and SNAS comparative data .Results Relative expanded measurement uncertainty of the above mentioned index by u-sing IQC data associated with PT data was the following :WBC(10 .02% ,7 .24% ,7 .04% ,from level 1 to level 3 respectively) ,RBC (2 .40% ,1 .72% ,1 .92% ,from level 1 to level 3 respectively) ,Hb(3 .54% ,2 .56% ,2 .50% ,from level 1 to level 3 respectively) , HCT(4 .12% ,3 .18% ,2 .86% ,from level 1 to level 3 respectively) ,PLT(15 .36% ,8 .86% ,7 .94% ,from level 1 to level 3 respec-tively) .Relative expanded measurement uncertainty of the above mentioned index by using IQC data associated with SNCS compar-ative data was the following :WBC(11 .66% ,7 .34% ,6 .40% ,from level 1 to level 3 respectively) ,RBC(2 .26% ,1 .60% ,1 .64%from level 1 to level 3 respectively) ,Hb(3 .36% ,2 .36% ,2 .10% ,from level 1 to level 3 respectively) ,HCT (3 .36% ,3 .04% , 3 .18% ,from level 1 to level 3 respectively) ,PLT (13 .34% ,8 .36% ,7 .14% ,from level 1 to level 3 respectively) .Conclusion Measurement uncertainty in complete blood cell could be estimated by using IQC data associated with SNCS comparative data , which is in accord with the instrument of target measurement uncertainty .
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Objective To explore effects of parents exposure to TBT on blood routine of F1 generation mice. Methods 80 mice including 40 males and 40 females, were randomly divided into control groups (CK) , low dose groups (LTBT), middle dose groups (MTBT) and high dose groups (HTBT).They were given dose of TBT (0,0.2,2, 20μg/kg) every day.The experiment lasted 45 days.At 60 days, one female and one male of the same concentration were bred in the same cage according to 1∶1.At postnatal day 60, blood was collected for the determination of blood routine. Results Compared with control group, the number of red blood cells and hemoglobin of F1 generation male mice in LTBT and HTBT groups were significantly increased (P <0.01); Red blood cell volume, mean corpuscular hemoglobin (P <0.01), and the lymphocyte absolute value in F1 generation male LTBT were significantly reduced (P <0.05); HTBT of female mice were significantly increased about the number of red blood cells (P <0.01).A dose-dependent increase of the hemoglobin, red blood cells, and platelet count of F1 generation female experimental groups was observed.Conclusion Parental TBT exposure affects the F1 mice blood routine.There is the greatest influence on LTBT in F1 generation male mice and on HTBT in F1 generation female mice.
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This study was aimed to discuss the anti-inflammatory and immunization properties ofRe-Du-Ning (RDN) injection in the treatment of acute exacerbation of chronic obstructive pulmonary disease (AECOPD) with phlegm-heat stagnated in the lung syndrome. A total of 110 in-patients were collected from January 2012 to December 2013. Cases were randomly divided into the treatment group (basic treatment plan + 20 ml RDN injection + 250 mL of 5% GS injection, once a day, intravenous injection) and the control group (basic treatment plan + 20 ml of 0.9% NS injection + 250 mL of 5% GS injection, once a day, intravenous injection), with 55 cases in each group. The treatment course was 14 days. Observations were made on traditional Chinese medicine (TCM) symptom score, clinical effect, blood routine examination, blood gas analysis and T lymphocyte subgroups before and after the treatment in both groups. The results showed that in the aspects of TCM symptom score and clinical effect, the posttreatment TCM symptom score and total integration in the treatment group were obviously improved compared to the control group except for shortness of breath, dry mouth and thirsty, flushing (P < 0.05). The total effective rate was obviously better than that of the control group. In the aspect of anti-inflammation, the total white blood cell (WBC) count and the normal neutrophil percentage of both pretreatment and posttreatment in the treatment group were obviously decreased compared to pretreatment (P < 0.05). However, there were no statistical differences on WBC count and normal neutrophil percentage compared to posttreatment in the control group. In the aspect of blood gas analysis, the posttreatment PaCO2 and PaO2 of the treatment group were obviously better than the control group (P < 0.05). In the aspect of immune regulation, the posttreatment T lymphocyte subgroups CD3+ and CD4+ of the treatment group were higher than the control group. The expression inhibition / cytotoxic lymphocyte (CD8+) was obviously lower than the control group (P < 0.05). The CD4+ / CD8+ was back to the normal reference level. It was concluded that in the treatment of AECOPD with phlegm-heat stagnated in the lung syndrome, on the basis of western medicine symptomatic treatment plan, RDN injection was assisted to clear heat, relieve toxin and remove phlegm. It can obviously improve patient’s clinical symptoms and increase the clinical therapeutic effects. The treatment was especially targeted to infection-induced respiratory failure patients combined low immunity with possible identified therapeutic effects.
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Objective To discuss the clinical significance of blood indicators in patients with fatty liver . Methods 122 patients with fatty liver were selected as the observation group ,and 131 patients with non-fatty liver were selected as the control group .All patients were given examinations of blood routine ,blood lipid,liver function. The differences of various blood indicators were compared ,and the relationship between liver function and blood lipid was analyzed.Results WBC,LYM,EO,RBC,HCT,HGB,MPV,P-LCR of the observation group were significantly higher than those of the control group ( t=10.694,3.584,5.427,18.65,22.857,23.014,4.217,4.684,all P<0.05),and PLT of the observation group was significantly lower than that of the control group (t=5.631,P<0.05). TG,TC,LDL-C,AST,ALT of observation group were significantly higher than those of the control group ( t=7.01, 8.45,7.26,13.62,11.98,all P<0.05),and HDL-C of the observation group was significantly lower than control group (t=4.13,P <0.05).ALT was positively correlated with TC,TG(OR =1.762,P <0.05),and negatively correlated with HDL-C ( OR=0 .7 4 5 , P<0 .0 5 ) .AST was positively correlated with ALT , TC ( OR=3 .6 7 8 , P<0 .0 5 ) , ALT and AST,TG,HDL-C had good linear relationship .Conclusion The blood indexes of patients with fatty liver and non-fatty liver exist significant differences , and the relationship between the indexes of liver function and blood lipids index is close ,and reducing blood fat levels can help to improve liver function .These results provide basis for the clinical diagnosis and guide therapy of fatty liver .
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Zhejiang province takes an innovative approach in its rural health service strategy featuring routine health examination,which proved an initial success.Intending to assess the effect and dilemma of such a system,and seek sustainable development in future,we made investigations in Pinhu and Xianju in Zhejiang province.Overall,rural health service strategy based on rural health routine examination,to some extent, improved patient-physician relationship,changed and strengthened their rural health concepts,and upgraded non-communicable disease control. Furthermore,the practice has economically and socially influenced rural health service institutions and rural physicians in terms of their concepts and methodology.There are also setbacks and problems in need of govemment support,programme improvement and resource optimization for further sustainability.